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Does Frederick Sanger's DNA sequencing technique remain in widespread use today?

  • Yes

  • No

  • Only in certain research areas

  • Only in combination with newer technologies

Answer

Frederick Sanger's dideoxy chain-termination method of DNA sequencing remains in widespread use today because it is a relatively straightforward and reliable method for determining the order of nucleotides in a DNA molecule. The method is based on the principle that when a DNA polymerase is synthesizing a DNA strand, it will incorporate a dideoxynucleotide (ddNTP) instead of a deoxynucleotide (dNTP) if a ddNTP is present in the reaction mixture.
Mastering the Life of Frederick Sanger: A Biochemical Quest

Mastering the Life of Frederick Sanger: A Biochemical Quest

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