Which technique did Sanger refine at the Laboratory of Molecular Biology?
The first-ever DNA sequencing technique
Protein folding technique
RNA replication technique
Cell culturing technique
Answer
The first-ever DNA sequencing technique was refined by Sanger at the Laboratory of Molecular Biology. Sanger's technique, known as the Sanger method, is a chain-termination method that uses dideoxynucleotides to terminate DNA synthesis and generate DNA fragments of known lengths. These fragments are then separated by electrophoresis and the resulting pattern of bands is used to determine the sequence of the DNA.
Mastering the Life of Frederick Sanger: A Biochemical Quest
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