Answer
In Beadle's experiment, X-rays were used to induce mutations in the genes of the fungus Neurospora crassa. By exposing the fungus to X-rays, he was able to observe the effects of mutations on the organism's phenotype, leading to the understanding that genes control specific biochemical reactions. This laid the foundation for elucidating the relationship between genes and enzymes, known as the "one gene-one enzyme" hypothesis, a crucial milestone in genetics.