Answer
Yes, Kirron Kher has portrayed negative roles in films. One such notable performance was in the 2006 movie Fanaa, where she played the role of the antagonist Mrs. Malhotra, a ruthless businesswoman and mother-in-law to the female lead, Zooni Ali Beg (played by Kajol). In this role, Kher displayed a cold and calculating demeanor, showcasing her versatility as an actor.