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Pee-wee Herman, the iconic character known for his childlike innocence and signature laugh, was developed by Paul Reubens with the assistance of Phil Hartman. Hartman, a fellow comedian and close friend of Reubens, provided valuable input and support during the creation of the character. Together, they crafted Pee-wee's unique mannerisms, wardrobe, and comedic style, which have made him a beloved figure in popular culture.