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Biochemistry is the field most impacted by Roger Y. Tsien's Nobel Prize-winning work. Tsien developed fluorescent proteins, which are used to visualize and study biological processes in living cells. This has revolutionized the field of biochemistry, allowing scientists to track and understand the dynamics of proteins in real time. The development of fluorescent proteins has also led to advances in other fields, such as cell biology, developmental biology, and neuroscience.